Rapid and Concerted Evolution of Repeat Units in a Balbiani Ring Gene

U. Lendahl ¹, H. Saiga ², C. Höög ¹, J. E. Edström ³, and L. Wieslander ¹

¹ Department of Molecular Genetics, Medical Nobel Institute, Karolinska Institutet, S-104 01 Stockholm, Sweden
² Department of Genetics, The Wallenberg Laboratory, University of Lund, S-220 07 Lund, Sweden, and Department of Molecular Biology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Kitakyushu, 807 Japan
³ Department of Genetics, The Wallenberg Laboratory, University of Lund, S-220 07 Lund, Sweden

The Balbiani ring (BR) genes in the midge Chironomus, a genus belonging to Diptera, code for large secretory proteins, used to construct the larval tube. The 15-23-kb long core block in each gene consists of an array of tandemly arranged approximately 200-bp long repeat units, where a single repeat unit is composed of a constant and a subrepeat region. In order to investigate the evolutionary fate of highly repetitive coding DNA, the BR1 core block in Chironomus pallidivittatus was characterized and compared to the orthologous core block in the sibling species Chironomus tentans. We find that the 75-100 repeat units in the BR1 core block have evolved in an unusual fashion. In all repeat units the constant regions display an expected high degree of homology between the two species, 94% at the nucleotide level. In contrast, the subrepeat regions in all repeat units have diverged concertedly, both as to length, number and sequence of the subrepeats. The observed changes in all repeat units of the core block probably have occurred after speciation of C. pallidivittatus and C. tentans. These findings demonstrate that a tandemly reiterated coding sequence can rapidly and concertedly convert into a related sequence, much in the same way as has been described for satellite DNA.