High Frequency Generalized Transduction by MiniMu Plasmid Phage

Boamin Wang ¹, Lin Liu ¹, Eduardo A. Groisman ², Malcolm J. Casadaban ², and Claire M. Berg ¹

¹ Department of Molecular and Cell Biology, U-131, The University of Connecticut, Storrs, Connecticut 06268
² Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637

Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.

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