Sequence of the Structural Gene for Xanthine Dehydrogenase ( rosy Locus) in Drosophila melanogaster

Tim P. Keith ¹, Margaret A. Riley ¹, Martin Kreitman ¹, R. C. Lewontin ¹, Daniel Curtis ², and Geoffrey Chambers ¹

¹ Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts 02138
² Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115

We determined the nucleotide sequence of a 4.6-kb EcoRI fragment containing 70% of the rosy locus. In combination with information on the 5' sequence, the gene has been sequenced in entirety. rosy cDNAs have been isolated and intron/exon boundaries have been determined. We find an open reading frame which spans four exons and would encode a protein of 1335 amino acids. The molecular weight of the encoded protein (xanthine dehydrogenase), based on the amino acid translation, is 146,898 daltons which agrees well with earlier biophysical estimates. Characteristics of the protein are discussed.

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