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First Evidence for Diploidy and Genetic Recombination in Free-Living Amoebae of the Genus Naegleria on the Basis of Electrophoretic Variation
Marie Louise Cariou 1 and Pierre Pernin 2
1 Laboratoire de Biologie et Génétique Evolutives,
Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France
2 Départment de Parasitologie, Faculté de Médecine
et de Pharmacie, 8 avenue Rockefeller, 69373 Lyon, France
Electrophoretic variation for 15 enzyme-coding genes was studied in various Naegleria (Rhizopoda, Vahlkampfiidae) species. The occurrence of complex banding patterns provided the first evidence of a diploid structure of the genome of these amoebae. The putative loci identified were found not to be linked and the genotypic distribution suggested chromosomal recombination for one species (Naegleria lovaniensis).
Submitted on July 22, 1986Accepted on October 22, 1986
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