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- Articles by Ennis, D. G.
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Genetic Functions Promoting Homologous Recombination in
Escherichia coli: A Study of Inversions in Phage 
Don G. Ennis 1, Susan K. Amundsen 2, and Gerald R. Smith 3
1 Institute of Molecular Biology, University of Oregon, Eugene,
Oregon 97403
2 Fred Hutchinson Cancer Research Center, 1124 Columbia Street,
Seattle, Washington 98104
3 Institute of Molecular Biology, University of Oregon, Eugene,
Oregon 97403, and Fred Hutchinson Cancer Research Center, 1124 Columbia Street,
Seattle, Washington 98104
We have studied homologous recombination in a derivative of phage 
containing two 1.4-kb repeats in inverted orientation. Inversion of the intervening
2.5-kb segment occurred efficiently by the Escherichia coli RecBC
pathway but markedly less efficiently by the Red pathway or the
E. coli RecE or RecF pathways. Inversion by the RecBCD pathway was stimulated
by Chi sites located to the right of the invertible segment; this stimulation
decreased exponentially by a factor of about 2 for each 2.2 kb between the
invertible segment and the Chi site. In addition to RecA protein and RecBCD
enzyme, inversion by the RecBC pathway required single-stranded DNA binding
protein, DNA gyrase, DNA polymerase I and DNA ligase. Inversion appeared to
occur either intra- or intermolecularly. These results are discussed in the
framework of a current molecular model for the RecBC pathway of homologous
recombination.
Accepted on September 13, 1986
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