- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Gruidl, M. E.
- Articles by Mosig, G.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Gruidl, M. E.
- Articles by Mosig, G.
SEQUENCE AND TRANSCRIPTS OF THE BACTERIOPHAGE T4 DNA REPAIR GENE uvsY
Michael E. Gruidl 1 and Gisela Mosig 1
1 Department of Molecular Biology, Vanderbilt University, Nashville,
Tennessee 37235
We have cloned, sequenced and analyzed transcription of the phage T4 uvsY gene. This gene is transcribed from a single gp MotA-dependent middle promoter to give a major transcript of approximately 930 nucleotides and a minor transcript of approximately 620 nucleotides. All in vivo and in vitro uvsY transcripts show anomalous migration in agarose gels. The uvsY transcript contains an open reading frame coding for an 137 amino acid [15.8 kilodaltons (kD)] UvsY protein and two unidentified open reading frames, ORF UvsY.-1 (9.0 kD) and ORF UvsY.-2 (6.0 kD). Our DNA sequence differs in only three places from that published by Takahashi et al. However, one of these changes alters the predicted carboxy terminus of the UvsY protein. Marker rescue experiments map gene 25 to the region upstream of uvsY. Gene 25 is likely, although not certain, to correspond to an ORF that is found upstream from uvsY and is translated in the same direction.
Submitted on June 23, 1986Accepted on August 15, 1986
This article has been cited by other articles:
 |
|
 |
|
  E. S. Miller, E. Kutter, G. Mosig, F. Arisaka, T. Kunisawa, and W. Ruger Bacteriophage T4 Genome Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 86 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Vaiskunaite, A. Miller, L. Davenport, and G. Mosig Two New Early Bacteriophage T4 Genes, repEA and repEB, That Are Important for DNA Replication Initiated from Origin E J. Bacteriol., November 15, 1999; 181(22): 7115 - 7125. [Abstract] [Full Text]
|
|