GENETIC ANALYSIS OF A MOUSE t COMPLEX LOCUS THAT IS HOMOLOGOUS TO A KIDNEY cDNA CLONE

Elizabeth A. Mann ¹, Lee M. Silver ², and Rosemary W. Elliott ¹

¹ Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263
² Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

A mouse kidney cDNA clone, pMK174, identifies restriction fragment length polymorphisms (RFLPs) that map to two unlinked loci. One, designated D17Rp17, has been mapped near quaking, (qk), on chromosome 17 using three sets of recombinant inbred (RI) strains. A study of several t haplotypes resulted in the identification of t-specific alleles of D17Rp17 that map to the proximal half of the t complex. Neither t-specific nor wild-type D17Rp17 alleles are present in chromosomes carrying either the T Orleans (Tt^Orl) or the T hairpin tail (T^hp) deletions. Comparison with other molecular markers indicates that pMK174 identifies a new proximal t complex locus, Rp17. The second locus identified by pMK174, termed D4Rp18, is tentatively assigned to chromosome 4 by mouse–Chinese hamster somatic cell hybrid analysis.

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