RECOMBINATION BETWEEN IS5 ELEMENTS: REQUIREMENT FOR HOMOLOGY AND RECOMBINATION FUNCTIONS

Michael S. Timmons ¹, M. Lieb ², and Richard C. Deonier ¹

¹ Molecular Biology, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-1481
² Department of Microbiology, University of Southern California School of Medicine, Los Angeles, California 90033

Intermolecular recombination between two IS5 elements was measured, using bacteriophage lambda recombination vectors, and was compared to recombination between two copies of an SV40 segment cloned into the same vectors. Experiments were conducted in the presence and in the absence of RecA and Red functions, and with the recombining inserts in the same or in reversed orientation. Under all conditions, IS5 elements recombined in a manner similar to the SV40 inserts, indicating that IS-encoded functions did not confer measurable additional intermolecular recombination ability to IS5 in E. coli K-12. Bacteriophages containing reversed IS5 inserts, for which the 16 base pair (bp) termini are identical in 15 positions and which display 12 bp of uninterrupted homology, recombined at approximately the same low frequency under Rec⁺ and Rec ^- conditions, indicating that these short homologies were not good substrates for the Rec system. Bacteriophages having reversed inserts recombined better under Red⁺ than under Red^- conditions, but the crossovers were located in nonhomologous regions flanking the element termini. This suggests that 12-bp homologies are not good substrates for the Red system.

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