INTERSTRAIN VARIATION IN AMYLASE GENE COPY NUMBER AND mRNA ABUNDANCE IN THREE MOUSE TISSUES

Miriam H. Meisler ¹, Tammy K. Antonucci ¹, Laurelee O. Treisman ¹, Deborah L. Gumucio ¹, and Linda C. Samuelson ¹

¹ Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, Michigan 48109

Amylase expression in strain YBR differs in several respects from the standard mouse phenotype. The synthesis of salivary amylase is elevated twofold in YBR mice and the synthesis of pancreatic amylase is reduced to one-half the normal rate. We have compared the concentrations of amylase mRNA in the parotid, liver and pancreas of YBR mice with those in strains A/J and C3H. We observed differences in amylase mRNA abundance which can account for the levels of amylase protein synthesis in the parotid and pancreas of these strains. Unexpectedly, the concentration of amylase mRNA in the liver of YBR mice was also higher than in the other strains. Since liver amylase is transcribed from the same gene as parotid amylase, duplication of the Amy-1 locus could account for the elevated mRNA concentration in both tissues. Quantitative analysis of genomic DNA by Southern blotting provided direct evidence for duplication of Amy-1 in strain YBR.