- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Blanco, C.
- Articles by Mata-Gilsinger, M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Blanco, C.
- Articles by Mata-Gilsinger, M.
NEGATIVE DOMINANT MUTATIONS OF THE uidR GENE IN ESCHERICHIA COLI: GENETIC PROOF FOR A COOPERATIVE REGULATION OF uidA EXPRESSION
Carlos Blanco 1, Paul Ritzenthaler 1, and Mireille Mata-Gilsinger 1
1 Laboratoire de Microbiologie de l'Institut National des Sciences
Appliquées de Lyon, Bâtiment 406, 20 Avenue Albert Einstein,
69621 Villeurbanne Cedex, France
The uidA gene is the first gene involved in the hexuronide-hexuronate pathway in Escherichia coli K-12 and is under the dual control of the uidR and uxuR encoded repressors. Point mutations affecting the uidR regulatory gene were sought to investigate the regulation of uidA. When the uidR mutant allele was on a multicopy plasmid and the wild-type allele was on the chromosome, some of the mutant phenotypes were dominant to the wild-type phenotype, indicating that the active form of the UidR repressor is multimeric. We have demonstrated that expression of the mutant phenotype is dependent on gene dosage. The dominance of the uidR allele was also sensitive to the presence of the wild-type uxuR allele in the cell. This behavior probably results from UidR-UxuR repressor interactions. A mechanism is proposed: we suggest that the UidR and UxuR repressors interact after their binding to the operator site of uidA; the binding of one regulatory molecule may facilitate the binding of the other one in a cooperative process.
Submitted on July 16, 1985Accepted on October 3, 1985
This article has been cited by other articles:
 |
|
 |
|
  J. L. Ramos, M. Martinez-Bueno, A. J. Molina-Henares, W. Teran, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes The TetR Family of Transcriptional Repressors Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 326 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Peekhaus and T. Conway What's for Dinner?: Entner-Doudoroff Metabolism in Escherichia coli J. Bacteriol., July 15, 1998; 180(14): 3495 - 3502. [Full Text]
|
|