- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Klar, A. J. S.
- Articles by Miglio, L. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Klar, A. J. S.
- Articles by Miglio, L. M.
SUM1, AN APPARENT POSITIVE REGULATOR OF THE CRYPTIC MATING-TYPE LOCI IN SACCHAROMYCES CEREVISIAE
Amar J. S. Klar 1, Satya N. Kakar 1, John M. Ivy 1, James B. Hicks 1, George P. Livi 1, and Lisa M. Miglio 1
1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
11724
The mating-type information residing at the HML and
HMR loci in Saccharomyces cerevisiae is kept unexpressed by
the action of at least four MAR (or SIR) loci. To determine
possible interactions between the MAR/SIR gene products and to find
new regulatory loci, we sought extragenic suppressors of the mar1-1 mutation.
A strain with the genotype HMLa MAT
HMR
a mar1-1 is unable to mate because of the simultaneous expression
of a and information. A mutant of this strain was isolated
that exhibits an phenotype and, therefore, presumably fails to express
the HML and HMR loci. We designate the new locus SUM1
(supressor of mar). The mutation is recessive, centromere
unlinked and does not correspond to the MAT, HML, HMR, SIR1, MAR1, MAR2
(SIR3) or SIR4 loci. The sum1 mutation affects
expression of both a and information at the HM loci.
Suppression by sum1-1 is neither allele specific nor locus specific
as it suppresses a deletion mutation of the MAR1 locus and mutations
in SIR3 and SIR4. The sum1-1 mutation has no discernible
phenotype in a Mar+ strain. We propose that the MAR/SIR gene
produts negatively regulate the SUM1 locus, the gene product of which
is necessary for expression of the HM loci.
Accepted on August 30, 1985
This article has been cited by other articles:
 |
|
 |
|
  O. A. Zill and J. Rine Interspecies variation reveals a conserved repressor of {alpha}-specific genes in Saccharomyces yeasts Genes & Dev., June 15, 2008; 22(12): 1704 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Safi, K. A. Wallace, and L. N. Rusche Evolution of New Function through a Single Amino Acid Change in the Yeast Repressor Sum1p Mol. Cell. Biol., April 15, 2008; 28(8): 2567 - 2578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Valenzuela, S. Gangadharan, and R. T. Kamakaka Analyses of SUM1-1-Mediated Long-Range Repression Genetics, January 1, 2006; 172(1): 99 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Lynch, H. B. Fraser, E. Sevastopoulos, J. Rine, and L. N. Rusche Sum1p, the Origin Recognition Complex, and the Spreading of a Promoter-Specific Repressor in Saccharomyces cerevisiae Mol. Cell. Biol., July 15, 2005; 25(14): 5920 - 5932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. McCord, M. Pierce, J. Xie, S. Wonkatal, C. Mickel, and A. K. Vershon Rfm1, a Novel Tethering Factor Required To Recruit the Hst1 Histone Deacetylase for Repression of Middle Sporulation Genes Mol. Cell. Biol., March 15, 2003; 23(6): 2009 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pak and J. Segall Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae Mol. Cell. Biol., September 15, 2002; 22(18): 6417 - 6429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pak and J. Segall Role of Ndt80, Sum1, and Swe1 as Targets of the Meiotic Recombination Checkpoint That Control Exit from Pachytene and Spore Formation in Saccharomyces cerevisiae Mol. Cell. Biol., September 15, 2002; 22(18): 6430 - 6440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. N. Rusche, A. L. Kirchmaier, and J. Rine Ordered Nucleation and Spreading of Silenced Chromatin in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2002; 13(7): 2207 - 2222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sousa, M. M. McLaughlin, S. A. Pereira, S. VanHorn, R. Knowlton, J. R. Brown, R. O. Nicholas, and G. P. Livi The ARO4 gene of Candida albicans encodes a tyrosine-sensitive DAHP synthase: evolution, functional conservation and phenotype of Aro3p-, Aro4p-deficient mutants Microbiology, May 1, 2002; 148(5): 1291 - 1303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe Nucleic Acids Res., April 1, 2002; 30(7): 1465 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sutton, R. C. Heller, J. Landry, J. S. Choy, A. Sirko, and R. Sternglanz A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex Mol. Cell. Biol., May 15, 2001; 21(10): 3514 - 3522. [Abstract] [Full Text]
|
|
|
|
|
|
L. N. R. a. J. Rine Conversion of a gene-specific repressor to a regional silencer Genes & Dev., April 15, 2001; 15(8): 955 - 967. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Dula and S. G. Holmes MGA2 and SPT23 Are Modifiers of Transcriptional Silencing in Yeast Genetics, November 1, 2000; 156(3): 933 - 941. [Abstract] [Full Text]
|
|
|
|
|
|
J. S. Smith, E. Caputo, and J. D. Boeke A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors Mol. Cell. Biol., April 1, 1999; 19(4): 3184 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Singer, A. Kahana, A. J. Wolf, L. L. Meisinger, S. E. Peterson, C. Goggin, M. Mahowald, and D. E. Gottschling Identification of High-Copy Disruptors of Telomeric Silencing in Saccharomyces cerevisiae Genetics, October 1, 1998; 150(2): 613 - 632. [Abstract] [Full Text]
|
|
|
|
|
|
C F Hardy, L Sussel, and D Shore A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes & Dev., May 1, 1992; 6(5): 801 - 814. [Abstract] [PDF]
|
|