- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Ernst, J. F.
- Articles by Sherman, F.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Ernst, J. F.
- Articles by Sherman, F.
DNA SEQUENCES OF FRAMESHIFT AND OTHER MUTATIONS INDUCED BY ICR-170 IN YEAST
Joachim F. Ernst 1, D. Michael Hampsey 1, and Fred Sherman 2
1 Department of Radiation Biology and Biophysics, University
of Rochester School of Medicine and Dentistry, Rochester, New York 14642
2 Department of Radiation Biology and Biophysics and Department
of Biochemistry, University of Rochester School of Medicine and Dentistry,
Rochester, New York 14642
ICR-170-induced mutations in the CYC1 gene of the yeast Saccharomyces cerevisiae were investigated by genetic and DNA sequence analyses. Genetic analysis of 33 cyc1 mutations induced by ICR-170 and sequence analysis of eight representatives demonstrated that over one-third were frameshift mutations that occurred at one site corresponding to amino acid positions 29–30, whereas the remaining mutations were distributed more-or-less randomly, and a few of these were not frameshift mutations. The sequence results indicate that ICR-170 primarily induces G·C additions at sites containing monotonous runs of three G·C base pairs. However, some (see PDF) sites within the CYC1 gene were not mutated by ICR-170. Thus, ICR-170 is a relatively specific mutagen that preferentially acts on certain sites with monotonous runs of G·C base pairs.
Submitted on April 27, 1985Accepted on June 14, 1985
This article has been cited by other articles:
 |
|
 |
|
  W.-D. Chen, J. R. Eshleman, M. R. Aminoshariae, A.-H. Ma, N. Veloso, S. D. Markowitz, W. D. Sedwick, and M. L. Veigl Cytotoxicity and Mutagenicity of Frameshift-Inducing Agent ICR191 in Mismatch Repair-Deficient Colon Cancer Cells J Natl Cancer Inst, March 15, 2000; 92(6): 480 - 485. [Abstract] [Full Text] [PDF]
|
|