RAD52-INDEPENDENT MITOTIC GENE CONVERSION IN SACCHAROMYCES CEREVISIAE FREQUENTLY RESULTS IN CHROMOSOMAL LOSS

James E. Haber ¹ and Mark Hearn ²

¹ Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254
² Department of Biology, Brandeis University, Waltham, Massachusetts 02254

We have examined spontaneous, interchromosomal mitotic recombination events between his4 alleles in both Rad⁺ and rad52 strains of Saccharomyces cerevisiae. In Rad⁺ strains, 74% of the His⁺ prototrophs resulted from gene conversion events without exchange of flanking markers. In diploids homozygous for the rad52-1 mutation, the frequency of His⁺ prototroph formation was less than 5% of the wild-type value, and more than 80% of the gene conversion events were accompanied by an exchange of flanking markers. Most of the rad52 intragenic recombination events arose by gene conversion accompanied by an exchange of flanking markers and not by a simple reciprocal exchange between the his4A and his4C alleles. There were also profound effects on the kinds of recombinant products that were recovered. The most striking effect was that RAD52-independent mitotic recombination frequently results in the loss of one of the two chromosomes participating in the gene conversion event.

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