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MAPPING CDC MUTATIONS IN THE YEAST S. Cerevisiae BY RAD52-MEDIATED CHROMOSOME LOSS
Pamela J. Hanic-Joyce 1
1 Department of Microbiology, Dalhousie University, Halifax,
Nova Scotia, Canada
Using the chromosome loss-mapping method of Schild and Mortimer, I have mapped several new temperature-sensitive mutations that define five CDC genes. Modified procedures were used to facilitate mapping temperature-sensitive mutations in general, and these modifications are discussed. The mutations were assigned to specific chromosomes by chromosome loss procedures, and linkage relationships were determined subsequently by standard tetrad analysis. Four of the mutations define new loci. The fifth mutation, cdc63-1, is shown to be allelic to previously known mutations in the PRT1 gene.
Submitted on October 30, 1984Accepted on April 5, 1985
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