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MOLECULAR CLONING OF 
-AMYLASE GENES FROM DROSOPHILA
MELANOGASTER. II. CLONE ORGANIZATION AND VERIFICATION
Jack N. Levy 1, Robert M. Gemmill 1, and Winifred W. Doane 1
1 Department of Zoology, Arizona State University, Tempe, Arizona
85287
Restriction maps of an 
-amylase structural gene clone, 
Dm65,
and of four putative -amylase pseudogene clones are presented. Two -amylase
structural genes, inverted with respect to each other, are contained in Dm65.
Subregions of internal DNA sequence homology within Dm65 and of cross-homology
between the presumptive pseudogene clones and Dm65 were determined.
Subregions of cross-homology between the Drosophila clones and the mouse -amylase
cDNA clone, pMSa104, were also determined. The presence of functional -amylase
structural genes in Dm65 was verified by injection of appropriate
subclones into the germinal vesicle of Xenopus oocytes, followed by incubation
of the oocytes under conditions that allowed coupled transcription and translation
of injected genes to occur. Subclones of the 3.8- and 5.6- kb EcoRI
fragments of Dm65 were shown to code for -amylase isozymes
1 and 3, respectively, of Drosophila melanogaster Canton-S. Both
subclones are homologous to RNA of a size sufficient to accommodate the -amylase-coding
information. No RNA species homologous to other subcloned EcoRI fragments
of Dm65 was detected.
Accepted on February 19, 1985
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