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MOLECULAR CLONING OF 
-AMYLASE GENES FROM DROSOPHILA
MELANOGASTER. I. CLONE ISOLATION BY USE OF A MOUSE PROBE
Robert M. Gemmill 1, Jack N. Levy 1, and Winifred W. Doane 1
1 Department of Zoology, Arizona State University, Tempe, Arizona
85287
A cloned 
-amylase cDNA sequence from the mouse is homologous
to a small set of DNA sequences from Drosophila melanogaster under
appropriate conditions of hybridization. A number of recombinant lambda phage
that carry homologous Drosophila genomic DNA sequences were isolated using
the mouse clone as a hybridization probe. Putative amylase clones hybridized
in situ to one or the other of two distinct sites in polytene chromosome
2R and were assigned to one of two classes, A and B. Clone 
Dm32,
representing class A, hybridizes within chromosome section 53CD. Clone Dm65
of class B hybridizes within section 54A1-B1. Clone Dm65 is homologous
to a 1450- to 1500-nucleotide RNA species, which is sufficiently long to code
for -amylase. No RNA homologous to Dm32 was detected. We suggest
that the class B clone, Dm65, contains the functional Amy structural
gene(s) and that class A clones contain an amylase pseudogene.
Accepted on February 19, 1985
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