GENETIC MAPPING OF CAENORHABDITIS ELEGANS COLLAGEN GENES USING DNA POLYMORPHISMS AS PHENOTYPIC MARKERS

George N. Cox ¹, Stephen Carr ¹, James M. Kramer ¹, and David Hirsh ¹

¹ Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309

In Caenorhabditis elegans collagens comprise a dispersed family of 40–150 genes, the majority of which probably code for collagen proteins found in the animal's cuticle. The conserved (Gly-X-Y)_n triple helix coding sequence of collagen genes has facilitated the isolation of a large number of C. elegans collagen genes by recombinant DNA methods. We have begun a study of the chromosomal organization of these genes by screening laboratory strains of C. elegans for DNA polymorphisms in the regions surrounding collagen genes. Polymorphisms near seven genes have been identified and have been used as phenotypic markers in genetic crosses to assign the genes to linkage groups II, III, IV, and X. Four genes are shown by multifactor crosses to map to a 2–3 map unit interval between unc-24 and unc-22 on chromosome IV.

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