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DOES CHI GIVE OR TAKE?
Franklin W. Stahl 1, M. Lieb 1, and Mary M. Stahl 1
1 Institute of Molecular Biology, University of Oregon, Eugene,
Oregon 97403
In lytic cycle crosses with Red-Gam-
phage, particles were examined that had undergone an Int-mediated
exchange. It was assumed that this exchange dimerized the circular
,
making it packageable. Among these Int-mediated recombinants, particles were
identified that had, in addition, enjoyed a close double exchange mediated
by the RecBC pathway. Such close double exchanges indicate localized negative
interference and are analogous to eukaryotic conversions that have retained
parental configuration of flanking markers. These events are stimulated by
Chi, a recombinator specific to the RecBC pathway. When Chi is present in
only one parent in the cross, the complementary double exchange recombinants
are Chi stimulated to the same degree. This behavior of Chi contrasts with
that of characterized eukaryotic recombinators.
Accepted on August 20, 1984
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