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SPECIFIC DELETION OCCURRING IN THE DIRECTED EVOLUTION OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ESCHERICHIA COLI
Raymond D. Miller 1, Daniel E. Dykhuizen 1, Louis Green 1, and Daniel L. Hartl 1
1 Department of Genetics, Washington University School of Medicine,
St. Louis, Missouri 63110
A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5 ) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.
Submitted on April 24, 1984Accepted on July 30, 1984
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