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ORGANIZATION AND MAPPING OF A SEQUENCE ON THE DROSOPHILA MELANOGASTER X AND Y CHROMOSOMES THAT IS TRANSCRIBED DURING SPERMATOGENESIS
Kenneth J. Livak 1
1 Department of Biology, University of California at San Diego,
La Jolla, California 92093
The D. melanogaster DNA segment in the recombinant phage 
Dm2L1
contains at least eight copies of a tandemly repeated 1250-base pair (bp)
sequence (henceforth called the 2L1 sequence). Testes from XO D. melanogaster
males contain an abundant 800-base RNA species that is homologous to
a 520-bp region of the 2L1 sequence. Blotting experiments show that the 2L1
sequence is repeated in the D. melanogaster genome and is present
on both the X and Y chromosomes. With the use of X-Y
translocations, the 2L1 sequence has been mapped to a region between
kl-1 and kl-2 on the long arm of the Y chromosome.
In Oregon-R wild type there are an estimated 200 copies of the 2L1 sequence
on the X chromosome and probably at least 80 copies on the Y
chromosome. In some other strains the repetition frequency on the
Y chromosome is about the same, but the copy number on the X chromosome
is much reduced. On the basis of the five strains investigated, there is a
correlation between copy number of the 2L1 sequence on the X chromosome
and the presence of a particular allele of the Stellate locus (Ste;
1-45.7). It seems that low copy number corresponds to Ste
+ and high copy number corresponds to Ste. The Ste locus
determines whether single or star-shaped crystals are observed in the spermatocytes
of XO males. Studies using D. simulans and D. mauritiana
DNA show that the 2L1 sequence is homologous to restriction fragments
in male DNA but not female DNA, indicating that this sequence is present only
on the Y chromosome in these two species. In DNA derived from
D. erecta, D. teissieri and D. yakuba, there is very little,
if any, hybridization with the 2L1 sequence probe.
Accepted on April 2, 1984
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