- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Conkling, M. A.
- Articles by Drake, J. W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Conkling, M. A.
- Articles by Drake, J. W.
ISOLATION AND CHARACTERIZATION OF CONDITIONAL ALLELES OF BACTERIOPHAGE T4 GENES uvsX AND uvsY
Mark A. Conkling 1 and John W. Drake 1
1 Laboratory of Genetics, National Institute of Environmental
Health Sciences, Research Triangle Park, North Carolina 27709
The bacteriophage T4 uvsW, uvsX and uvsY gene functions are required for wild-type levels of recombination and for normal survival and mutagenesis after treatments with ultraviolet (UV) and ionizing radiations. The ability of uvsX and uvsY mutations to suppress the lethality of gene 49 mutations was used to select temperature-sensitive and amber alleles of these two genes. (uvsW mutations do not suppress gene 49 mutations.) A simple and powerful complementation test was developed to assist in assigning uvs mutations to genes. The amber alleles of uvsX and uvsY behave as simple null alleles, fully suppressing a gene 49 defect, enhancing UV killing and abolishing UV mutagenesis. However, the properties of the ts alleles of uvsX and uvsY demonstrated that suppression of a gene 49 defect, sensitivity to UV-induced inactivation and UV mutability can be partially uncoupled. These results prompt the hypothesis that radiation mutagenesis occurs during DNA chain elongation past template damage within a recombinational intermediate rather than within a conventional replication fork.
Submitted on October 14, 1983Accepted on March 22, 1984
This article has been cited by other articles:
 |
|
 |
|
  S. W. Nelson and S. J. Benkovic The T4 Phage UvsW Protein Contains Both DNA Unwinding and Strand Annealing Activities J. Biol. Chem., January 5, 2007; 282(1): 407 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Miller, E. Kutter, G. Mosig, F. Arisaka, T. Kunisawa, and W. Ruger Bacteriophage T4 Genome Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 86 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Dressman and J. W. Drake Lysis and Lysis Inhibition in Bacteriophage T4: rV Mutations Reside in the Holin t Gene J. Bacteriol., July 15, 1999; 181(14): 4391 - 4396. [Abstract] [Full Text]
|
|
|
|
|
|
A. Bebenek, L. A. Smith, and J. W. Drake Bacteriophage T4 rnh (RNase H) Null Mutations: Effects on Spontaneous Mutation and Epistatic Interaction with rII Mutations J. Bacteriol., May 15, 1999; 181(10): 3123 - 3128. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. A. Smith and J. W. Drake Aspects of the Ultraviolet Photobiology of Some T-Even Bacteriophages Genetics, April 1, 1998; 148(4): 1611 - 1618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Dressman, C.-C. Wang, J. D. Karam, and J. W. Drake Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment PNAS, July 22, 1997; 94(15): 8042 - 8046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bebenek, H. K. Dressman, G. T. Carver, S.-s. Ng, V. Petrov, G. Yang, W. H. Konigsberg, J. D. Karam, and J. W. Drake Interacting Fidelity Defects in the Replicative DNA Polymerase of Bacteriophage RB69 J. Biol. Chem., March 23, 2001; 276(13): 10387 - 10397. [Abstract] [Full Text] [PDF]
|
|