ISOLATION AND CHARACTERIZATION OF NEUROSPORA MUTANTS AFFECTED IN INVERTASE SYNTHESIS

Deborah B. Lee ¹ and Stephen J. Free ¹

¹ Division of Cell and Molecular Biology, Department of Biological Sciences, SUNY/Buffalo, Buffalo, New York 14260

We have outlined a procedure that allows the large-scale screening of mutagenized Neurospora crassa populations for invertaseless mutants. We have isolated and characterized three mutations, inv(DBL1), inv(DBL9) and inv(DBL14), which have been mapped at or near the invertase structural gene. One of these, inv(DBL1), is particularly interesting. Our experiments indicate that the reduced level of invertase activity in the inv(DBL1)-containing cell can be explained as the result of a reduced number of normal enzyme molecules. We also show that wild-type Neurospora is able to respond rapidly to a change of medium and can dramatically increase its production of invertase within 20 min after a transfer to a carbon-free medium.