- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Clegg, M. T.
- Articles by Thomas, K.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Clegg, M. T.
- Articles by Thomas, K.
CHLOROPLAST DNA VARIATION IN PEARL MILLET AND RELATED SPECIES
Michael T. Clegg 1, James R. Y. Rawson 1, and Karen Thomas 1
1 Departments of Botany and Molecular and Population Genetics,
University of Georgia, Athens, Georgia 30602
The evolution of specific regions of the chloroplast genome was studied in five grass species in the genus Pennisetum, including pearl millet, and one species from a related genus (Cenchrus). Three different regions of the chloroplast DNA were investigated. The first region included a 12-kilobase pair (kbp) EcoRI fragment containing the 23S, 16S and 5S ribosomal RNA genes, which is part of a larger duplicated region of reverse orientation. The second region was contained in a 21-kbp Sa/I fragment, which spans the short single-copy sequence separating the two reverse repeat structures and which overlaps the duplicated copies of the 12-kbp Eco RI fragment. The third region was a 6-kbp EcoRI fragment located in the large single-copy region of the chloroplast genome. Together these regions account for slightly less than 25% of the chloroplast genome. Each of these DNA fragments was cloned and used as hybridization probes to determine the distribution of homologous DNA fragments generated by various restriction endonuclease digests.—A survey of 12 geographically diverse collections of pearl millet showed no indication of chloroplast DNA sequence polymorphism, despite moderate levels of nuclear-encoded enzyme polymorphism. Interspecific and intergeneric differences were found for restriction endonuclease sites in both the small and the large single-copy regions of the chloroplast genome. The reverse repeat structure showed identical restriction site distributions in all materials surveyed. These results suggest that the reverse repeat region is differentially conserved during the evolution of the chloroplast genome.
Submitted on August 2, 1983Accepted on November 16, 1983
This article has been cited by other articles:
 |
|
 |
|
  O. Gulsen, R. C. Shearman, K. P. Vogel, D. J. Lee, and T. Heng-Moss Organelle DNA Diversity among Buffalograsses from the Great Plains of North America Determined by cpDNA and mtDNA RFLPs Crop Sci., January 1, 2005; 45(1): 186 - 192. [Abstract] [Full Text] [PDF]
|
|