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THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA- recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA
Ichizo Kobayashi 1, Mary M. Stahl 1, David Leach 1, and Franklin W. Stahl 1
1 Institute of Molecular Biology, University of Oregon, Eugene,
Oregon 97403
Chi (5'-GCTGGTGG) is a recombinator in RecA- RecBC-mediated
recombination in Escherichia coli. In bacteriophage 
vegetative
recombination, Chi is fully active only when it is correctly oriented with
respect to cos, the site that defines the ends of the packaged chromosome.
Here we demonstrate that packaging from cos is not necessary for
this cos-Chi interaction. Our evidence suggests that correctly oriented
cos is an activator of Chi. cos, as an activator, is (1) dominant
over cos-, (2) active opposite an extensive heterology,
(3) able to interact with Chi only when on the same (cis) chromosome,
and (4) able to interact with Chi at distances as far as 
20 kb. Thus,
cos and Chi form a two-component recombinator system for general recombination.
cos may serve as an asymmetric entry site for a recombination enzyme
that recognizes Chi in an asymmetric way.
Accepted on April 22, 1983
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