LOCATION OF THE LSP-1 GENES IN DROSOPHILA SPECIES BY IN SITU HYBRIDIZATION

Hugh W. Brock ¹ and David B. Roberts ¹

¹ The Genetics Laboratory, Department of Biochemistry, Oxford University, Oxford, United Kingdom OX1 3QU

The locations of the larval serum protein one (LSP-1) , ß and genes were determined in Drosophila melanogaster and in 14 other species of Drosophila by in situ hybridization to polytene chromosomes. The LSP-1 gene mapped to bands 11B on the X chromosome, the LSP-1 ß gene mapped to bands 21D-E on chromosome 2L, and the LSP-1 gene mapped to band 61A in all the melanogaster subgroup species. In eight other species, both the LSP-1 and ß genes mapped to one site on Muller's element E which corresponds to chromosome 3R of D. melanogaster. No hybridization of LSP-1 was detected in these eight species. Restriction enzyme digestion and analysis of genomic DNA by filter transfer hybridization confirmed the presence of LSP-1 -like and ß-like genes in seven of these species. These results are discussed with respect to conservation of the chromosomal elements in the genus Drosophila.

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