INDUCTION OF MUTATIONS BY -RAYS IN PREGONIAL GERM CELLS OF ZEBRAFISH EMBRYOS

Charline Walker ¹ and George Streisinger ¹

¹ Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403

Specific locus (gol-1) germ line mutations are induced by -rays with high frequencies (about 10^-5 r ^-1) during cleavage divisions in zebrafish. Mutant clone sizes range from 3 to 50% of the total number of germ cells, with a mean of about 10%, when embryos are exposed between the 16 and 10³ cell stages. About five pregonial cells are calculated to be present during the cleavage period.

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