PEP4 GENE FUNCTION IS REQUIRED FOR EXPRESSION OF SEVERAL VACUOLAR HYDROLASES IN SACCHAROMYCES CEREVISIAE

Elizabeth W. Jones ¹, George S. Zubenko ¹, and Roy R. Parker ¹

¹ Department of Biological Sciences, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213

The pep4-3 mutation results in a 90–95% reduction in the levels of five vacuolar hydrolases in yeast, including proteinases A and B, carboxypeptidase Y, RNase(s) and the repressible alkaline phosphatase. The mutation is without effect on two secreted glycoproteins, on an enzyme of the vacuolar membrane, and on a proteinase located outside of the vacuole. Mutations at the PEP4 locus exhibit a dosage effect on the levels of some, but not all, of the enzymes whose expression requires the function of the gene.

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