CHI-STIMULATED RECOMBINATION BETWEEN PHAGE lambda AND THE PLASMID lambdadv

1 Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403

Chi promotes Rec-mediated recombination between phage lambda DNA and the homologous plasmid lambdadv. In the absence of Chi, some of the interactions splice lambdadv into lambda, whereas others patch information from lambdadv into lambda. When Chi is in the phage DNA, splices and patches are increased in frequency by the same factor. This result strengthens the analogy between Chi and recombination-promoting elements in fungi. It also rules out one model for the previously reported orientation dependence of Chi phenotype.

Submitted on July 2, 1982
Accepted on August 18, 1982




This article has been cited by other articles:


Home page
GeneticsHome page
S. K. Amundsen and G. R. Smith
Chi Hotspot Activity in Escherichia coli Without RecBCD Exonuclease Activity: Implications for the Mechanism of Recombination
Genetics, January 1, 2007; 175(1): 41 - 54.
[Abstract] [Full Text] [PDF]