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CHI-STIMULATED RECOMBINATION BETWEEN PHAGE
AND THE PLASMID
dv
Franklin W. Stahl 1, Mary M. Stahl 1, Lisa Young 1, and Ichizo Kobayashi 1
1 Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
Chi promotes Rec-mediated recombination between phage
DNA and the homologous plasmid
dv. In the absence of Chi, some of the interactions splice
dv into
, whereas others patch information from
dv into
. When Chi is in the phage DNA, splices and patches are increased in frequency by the same factor. This result strengthens the analogy between Chi and recombination-promoting elements in fungi. It also rules out one model for the previously reported orientation dependence of Chi phenotype.
Accepted on August 18, 1982
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