DETERMINATION OF THE ORDER OF GENE FUNCTION IN THE YEAST NUCLEAR DIVISION PATHWAY USING cs AND ts MUTANTS

Don Moir ¹ and David Botstein ¹

¹ Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Cold-sensitive (cs) and heat-sensitive (ts) conditional-lethal mutations that affect specifically the cell division cycle of budding yeast (Saccharomyces cerevisiae) were used to determine the order of gene function. Reciprocal temperature-shift experiments using cs-ts double mutants revealed a detailed order of function among genes whose execution points and mutant phenotypes are very similar. The data suggest that the nuclear branch of the overall cell-cycle pathway itself contains at least one branch.

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