FORMATION OF F'TRP PLASMIDS IN ESCHERICHIA COLI K12

1 Laboratoire de Génétique, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium

From strains carrying two different F-prime factors, we recovered F' derivatives that acquired the trp chromosomal region. These F'trp plasmids can be isolated at a frequency of 10-5 to 10-6. They were characterized genetically by looking at the size of the trp segment they acquired and at the location of that segment in the parental F' plasmid. Results are discussed in relationship to possible transposition mechanisms.

Submitted on July 23, 1981
Accepted on December 22, 1981